As with a traditional differential extraction, the epithelial cells are lysed and the sperm cells are pelleted. After removing the epithelial DNA for testing, the remaining epithelial cell DNA is destroyed while leaving the sperm cells intact. It is possible to destroy all of the epithelial DNA even when there are a relatively small number of sperm cells and overwhelming quantities of epithelial DNA. This method eliminates mixture profiles in nearly all cases.
Partially funded by NIJ Grant 2009-DN-BX-K039